s. mutans whole cell antiserum (Lampire Biological)
90
Structured Review
Lampire Biological
s. mutans whole cell antiserum
S. Mutans Whole Cell Antiserum, supplied by Lampire Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s%2E+mutans+whole+cell+antiserum/s++mutans+whole+cell+antiserum/pmc06141683-87-26-45
Average 90 stars, based on 1 article reviews
S. Mutans Whole Cell Antiserum, supplied by Lampire Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s%2E+mutans+whole+cell+antiserum/s++mutans+whole+cell+antiserum/pmc06141683-87-26-45
Average 90 stars, based on 1 article reviews
s. mutans whole cell antiserum - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation"
Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2018.02130
Figure Legend Snippet: The schematic diagram of the mecA flanking region (A) and RT-PCR analysis (B) . (A) Schematic diagram of the flanking regions of mecA in the genome of S. mutans UA159. Numbers underneath represent the size of the respective genes. Arrows above and underneath indicate the location and orientation of the primers used for reverse transcription (5) and cDNA amplification (F and R). (B) RT-PCR analysis of the mecA / rgpG cluster. cDNA generated from reverse transcription using total RNA extract of S. mutans was PCR amplified using primer set PmecARgpG (lane 1), which shows a single amplicon indicative of co-transcription of mecA and rgpG . Negative control (lane 2) with no reverse transcriptase shows no DNA amplification. M, for molecular marker.
Techniques Used: Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Amplification, Generated, Negative Control, DNA Amplification, Marker
Figure Legend Snippet: Colony morphology of the mecA mutant. S. mutans wild-type (UA159), the mecA mutant (TW416) and its complement strain (TW416C) were grown on BHI agar plates. Relative to the rough, dry colonies of UA159, the colonies of TW416 were round, mucoid, and smooth. Images were taken using Samsung Galaxy Note V.
Techniques Used: Mutagenesis
Figure Legend Snippet: Growth characterization of the mecA mutant. (A) Growth phenotypes of S. mutans wild-type (UA159), the mecA mutant (TW416) and its complement strain (TW416C) when grown in BHI broth overnight. Arrow indicates severe clumping of the mecA mutant at the tip of the tooth pick. (B) S. mutans strains grown in BHI broth, BHI broth with pH adjusted to 6.0 and BHI broth with inclusion of methyl viologen at 12.5 mM. The optical densities of the cultures at 600 nm were recorded continuously using a Bioscreen C. Data presented in panel (B) are representatives of three separate experiments.
Techniques Used: Mutagenesis
Figure Legend Snippet: TEM analysis. S. mutans wildtype (UA159 and A&D), the mecA mutant (TW416 and B&E) and its mecA complement strain (TW416C and C&F) were grown in BHI broth until mid-exponential phase (OD 600nm ≈0.4). (A,B,C) were images taken at magnification of 10,000 × g , and (D,EF) at 25,000×, respectively. Scale bars represent 500 nm. Images in (G) are inserts of blow-up regions of the cell envelope of the different strains with the mutant showing a fuzzy, loose cell envelope (ce) and a thin cytoplasmic membrane (cm), as indicated. The arrows in (E) indicate the fuzzy, loose cell envelope and the asterisks indicating low-density patches of the deficient mutant.
Techniques Used: Mutagenesis, Membrane
Figure Legend Snippet: Biofilm formation. S. mutans wildtype (UA159), the mecA mutant (TW416) and its complement strain (TW416C) were grown in BM medium with glucose and sucrose (BMGS), glucose (BMG) or sucrose (BMS). Biofilms were grown on polystyrene surface in 96 well plates and analyzed using a spectrophotometer. Results presented here represent mean absorbance at 575 nm (±standard deviation in error bars) from three independent experiments and ∗ and # P < 0.01, P < 0.05, respectively.
Techniques Used: Mutagenesis, Spectrophotometry, Standard Deviation
Figure Legend Snippet: Confocal microscopic analysis of biofilms. S. mutans wildtype (UA159), the mecA mutant (TW416) and its complement strain (TW416C) were grown in BM medium with glucose and sucrose, glucose or sucrose. Biofilms were grown on HA disks vertically placed in 12 well plates for 24 h, and analyzed using a laser scanning confocal microscope. Panel shows representatives of the compressed confocal images at xy, yz, and xz axis of biofilms of UA159, TW416, and TW416C grown in BM plus glucose and sucrose.
Techniques Used: Mutagenesis, Microscopy
Figure Legend Snippet: SEM analysis of biofilms. S. mutans wildtype (UA159), the mecA mutant (TW416) and its complement strain (TW416C) were grown in BM medium with glucose and sucrose, glucose or sucrose. Biofilms were grown on HA disks vertically placed in 12 well plates for 24 h, and analyzed using a scanning electron microscope (SEM). Panel shows images of UA159 and TW416 biofilms grown in BM plus glucose and sucrose, which were taken at magnification of 5,000 and 10,000 × g as indicated, with arrows indicating extracellular polymeric substances and asterisks indicating broken cells and cell debris, respectively.
Techniques Used: Mutagenesis, Microscopy
Figure Legend Snippet: Acid and hydrogen peroxide challenge assays. S. mutans strains were grown until mid-exponential phase (OD 600nm ≈0.3) and then subjected to an acid or hydrogen peroxide killing. (A) shows survival rate of wildtype (UA159), the mecA mutant (TW416) and its complement strain (TW416C) following incubation in glycin buffer of pH 2.8, while (B) shows survival rate of the strains in the presence of hydrogen peroxide. Data represented here are means (±standard deviation) of at least three independent experiments, with ∗ P < 0.001 as comparing to the wild-type.
Techniques Used: Mutagenesis, Incubation, Standard Deviation
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nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize Dot Blot:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation 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Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize Mutagenesis:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize Reverse Transcription Polymerase Chain Reaction:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize Reverse Transcription:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize Amplification:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize Negative Control:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize DNA Amplification:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize Marker:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize Spectrophotometry:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize Standard Deviation:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize Microscopy:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize Incubation:Article Title: Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans Article Snippet: 222 For immunoblot analysis, equal amount of the above antigen preps were blotted onto 223 a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad) (34), and probed with 224 on July 9, 2017 by U N IV O F C A LIF S A N D IE G O http://aem .asm .org/ D ow nloaded from 11 S. mutans whole cell antiserum that was generated using inactivated whole cells of the 225 wild-type, UA159 to immunize Article Title: Deficiency of MecA in Streptococcus mutans Causes Major Defects in Cell Envelope Biogenesis, Cell Division, and Biofilm Formation Article Snippet: For immunoblot analysis, equal amount of the above antigen preps were blotted onto a nitrocellulose membrane using a Slot Blot (Bio-Dot SF, BioRad), and probed with S. mutans whole cell antiserum that was generated using inactivated whole cells of the wild-type, UA159 to immunize |
![Biofilm formation. The S. mutans wild-type strain (UA159), its rgpG mutants (TW322 for the 96-well plate model and TW393 for HA discs), and the rgpG complement strain (TW393C) were grown in BM supplemented with glucose (BMG), sucrose (BMS), and glucose plus sucrose (BMGS). Biofilms were grown in 96-well plates (A) and HA discs vertically placed in 12-well plates (B and C) for 24 and 48 h. By the end of the experiments, biofilms on 96-well plates were stained with crystal violet and analyzed using a spectrophotometer (A); biofilms on HA discs were fixed overnight and processed for SEM analysis (B), or stained using the LIVE/DEAD fluorescent staining kit and examined by confocal microscopy (C). The results presented in panel A are averages (±standard deviation [SD] shown by error bars) of biofilms after 24 and 48 h from more than three independent experiments. * and # indicate statistically significant difference at P < 0.001 and P < 0.05, respectively, compared to the wild type. Panel B shows representative SEM images (at 5,000×) of 24-h biofilms grown in BM plus glucose and sucrose. Panel C shows representative compressed xyz, xz, and yz confocal microscopic images (512 by 512) of biofilms grown in BM with glucose and sucrose.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1293/pmc05561293/pmc05561293__zam999118035003a.jpg)
